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mouse anti mof  (Boster Bio)


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    Boster Bio mouse anti mof
    Mouse Anti Mof, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+mof/pmc09482892-174-38-40?v=Boster+Bio
    Average 91 stars, based on 4 article reviews
    mouse anti mof - by Bioz Stars, 2026-08
    91/100 stars

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    O -GlcNAc, O -GlcNAc transferase (OGT), MOF <t>(KAT8),</t> and ATM levels were elevated in aged aorta tissues. (A) Illustration of mouse aorta tissue. (B) Immunofluorescence staining of OGT (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity in young (6 months) and old (21 months) mouse aorta tissue. (C) Quantification of OGT mean fluorescence in young (n=39) and old mice (n=49). (D) Immunofluorescence staining of O -GlcNAc (red) and actin (green). (E) Quantification of O -GlcNAc mean fluorescence in young (n=63) and old mice (n=63). (F) Immunofluorescence staining of ATM (pS/Tq, red) and actin (green). (G) Quantification of ATM mean fluorescence in young (n=39) and old mice (n=53). (H) Immunofluorescence staining of H2AX (red) and actin (green). (I) Quantification of ATM mean fluorescence in young (n=56) and old mice (n=69). (J) Immunofluorescence staining of MOF (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity. (K) Quantification of MOF mean fluorescence in young (n=35) and old mice (n=45). The white line indicates the width of the aorta. The insert in each panel is an enlarged image of the area where the line is displayed. Data are presented as the mean±SD. **** P <0.0001. ns, non-significant. ATM, ataxia telangiectasia mutated.
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    O -GlcNAc, O -GlcNAc transferase (OGT), MOF <t>(KAT8),</t> and ATM levels were elevated in aged aorta tissues. (A) Illustration of mouse aorta tissue. (B) Immunofluorescence staining of OGT (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity in young (6 months) and old (21 months) mouse aorta tissue. (C) Quantification of OGT mean fluorescence in young (n=39) and old mice (n=49). (D) Immunofluorescence staining of O -GlcNAc (red) and actin (green). (E) Quantification of O -GlcNAc mean fluorescence in young (n=63) and old mice (n=63). (F) Immunofluorescence staining of ATM (pS/Tq, red) and actin (green). (G) Quantification of ATM mean fluorescence in young (n=39) and old mice (n=53). (H) Immunofluorescence staining of H2AX (red) and actin (green). (I) Quantification of ATM mean fluorescence in young (n=56) and old mice (n=69). (J) Immunofluorescence staining of MOF (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity. (K) Quantification of MOF mean fluorescence in young (n=35) and old mice (n=45). The white line indicates the width of the aorta. The insert in each panel is an enlarged image of the area where the line is displayed. Data are presented as the mean±SD. **** P <0.0001. ns, non-significant. ATM, ataxia telangiectasia mutated.
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    Image Search Results


    O -GlcNAc, O -GlcNAc transferase (OGT), MOF (KAT8), and ATM levels were elevated in aged aorta tissues. (A) Illustration of mouse aorta tissue. (B) Immunofluorescence staining of OGT (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity in young (6 months) and old (21 months) mouse aorta tissue. (C) Quantification of OGT mean fluorescence in young (n=39) and old mice (n=49). (D) Immunofluorescence staining of O -GlcNAc (red) and actin (green). (E) Quantification of O -GlcNAc mean fluorescence in young (n=63) and old mice (n=63). (F) Immunofluorescence staining of ATM (pS/Tq, red) and actin (green). (G) Quantification of ATM mean fluorescence in young (n=39) and old mice (n=53). (H) Immunofluorescence staining of H2AX (red) and actin (green). (I) Quantification of ATM mean fluorescence in young (n=56) and old mice (n=69). (J) Immunofluorescence staining of MOF (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity. (K) Quantification of MOF mean fluorescence in young (n=35) and old mice (n=45). The white line indicates the width of the aorta. The insert in each panel is an enlarged image of the area where the line is displayed. Data are presented as the mean±SD. **** P <0.0001. ns, non-significant. ATM, ataxia telangiectasia mutated.

    Journal: Preventive Nutrition and Food Science

    Article Title: Magnolia kobus DC. Regulates Vascular Smooth Muscle Cell Proliferation by Modulating O -GlcNAc and MOF Expression

    doi: 10.3746/pnf.2024.29.4.430

    Figure Lengend Snippet: O -GlcNAc, O -GlcNAc transferase (OGT), MOF (KAT8), and ATM levels were elevated in aged aorta tissues. (A) Illustration of mouse aorta tissue. (B) Immunofluorescence staining of OGT (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity in young (6 months) and old (21 months) mouse aorta tissue. (C) Quantification of OGT mean fluorescence in young (n=39) and old mice (n=49). (D) Immunofluorescence staining of O -GlcNAc (red) and actin (green). (E) Quantification of O -GlcNAc mean fluorescence in young (n=63) and old mice (n=63). (F) Immunofluorescence staining of ATM (pS/Tq, red) and actin (green). (G) Quantification of ATM mean fluorescence in young (n=39) and old mice (n=53). (H) Immunofluorescence staining of H2AX (red) and actin (green). (I) Quantification of ATM mean fluorescence in young (n=56) and old mice (n=69). (J) Immunofluorescence staining of MOF (red) and ATM (pS/Tq, green), which indicates ATM/ATR activity. (K) Quantification of MOF mean fluorescence in young (n=35) and old mice (n=45). The white line indicates the width of the aorta. The insert in each panel is an enlarged image of the area where the line is displayed. Data are presented as the mean±SD. **** P <0.0001. ns, non-significant. ATM, ataxia telangiectasia mutated.

    Article Snippet: The primary antibodies used in this study included mouse anti-OGT (C-10) (Cat# sc-376253, 1:100 dilution; Santa Cruz Biotechnology), mouse anti- O -linked N-acetylglucosamine ( O -GlcNAc) (HGAC85) (Cat# MA1-076, 1:100 dilution; Thermo Fisher Scientific), mouse anti-phospho-Histone H2A.X (Ser139) (Cat# 05-636, 1:300 dilution; Millipore), rabbit phospho-ATM/ATR Substrate Motif [(pS/pT) QG] (Cat# 6966S, 1:300 dilution; Cell Signaling), mouse anti-MOF (KAT8) (Cat# sc-271691, 1:100 dilution; Santa Cruz Biotechnology), rabbit anti-Ki-67 (Cat# ab15580, 1:100 dilution; Abcam), mouse anti-Ki-67 (SP6; Cat# MA5-14520, 1:50 dilution; Thermo Fisher Scientific), and rabbit anti-actin (Cell Signaling, 1:300 dilution).

    Techniques: Immunofluorescence, Staining, Activity Assay, Fluorescence